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KMID : 0359320030430030477
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Korean Journal of Veterinary Research
2003 Volume.43 No. 3 p.477 ~ p.483
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Rapid and Easy Detection of Porcine Epidemic Diarrhea Virus (PEDV) by in situ Hybridization
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Abstract
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Molecular diagnostic techniques have been used to identify porcine epidemic diarrhea virus (PEDA), a causative agent of acute enteritis is swine, but they were difficult to be performed and time-consuming. To detect PEDV in a rapid and easy way, we developed biotinylated cDNA probe for N gene encoding the nucleoproteins of PEDV. Formalin-fixed and paraffin-embedded tissues from 24 naturally infected pigs were used for the experiment,
The ISH produced a positive reaction in all cases. When intestinal tissues were hybridized with PEDV probe, strong signals were seen in the villus enterocytes of the jejunum and ileum. Hybridization signals were also found in the duodenurn from one pig and in colon from donther. In conclusion, ISH with a biotinylated cDNA probe was provided to be a useful diagnostic method for detecting PEDV effectively in routinely processed tissue sections.
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